Expression of Prostaglandin-Related Genes in Broilers Under the Influence of Glyphosate and Probiotic
Abstract. The presence of residues of the herbicide glyphosate in poultry feed is a fairly common problem. Our experiments were carried out in the vivarium of LLC "BIOTROF +" on broilers of the cross "Ross 308". For the experiment, the birds were divided into 3 groups: 1 control group, which received a diet without additives, diet with the addition of glyphosate and the strain of the microorganism Bacillus sp.GL-8.The analysis of gene expression of the caecum of the intestines of broilers was carried out using quantitative PCR with reverse transcription.For the analysis of mRNA expression, specific primers were selected for the following prostaglandin genes studied: PTGER3, PTGER4, PTGR1, PTGDS and PTGES Amplification reactions were performed using SsoAdvanced ™ Universal SYBR® Green Supermix (Bio-Rad) For the first time in our study, the impact of glyphosates on poultry occurs, including through changes in the activity of key genes associated with prostaglandin metabolism Glyphosate introduced into bird feed in an amount corresponding to I eat one MPC, acted as an inducer of prostaglandin receptor gene expression (PTGER3 and PTGER4), prostaglandin catabolism (PTGR1) and prostaglandin synthesis (PTGDS and PTGES) in the caeca of the intestine of broilers. Thus, the levels of mRNA expression of the PTGER3 gene and the PTGER4 gene when exposed to glyphosate (group 2) were activated 1.87 and 1.91 times compared with the control group 1, PTGDS and PTGES genes - 1.35 times, PTGR1 gene - 1 .87 times (P≤0.05). In the future, the PTGER3, PTGER4, PTGDS, and PTGR1 genes can be used as possible therapeutic targets for toxicosis caused, in particular, by feed glyphosates. The probiotic strain of Bacillus sp. GL-8 probably restored the microbiota, thereby enhancing its protective effect against toxicants, in particular, its metabolic functions of biodegradation. This was expressed in the “smoothing” of the level of PTGR1 gene activity to the level of the control group, as well as a decrease in the activity of PTGDS, PTGES, and PTGER3. Positive shifts in the change in transcription of prostaglandin genes under the influence of a strain of a probiotic microorganism indicate the prospect of using probiotics as a tool for leveling physiological imbalance against the background of feed contamination with toxic substances.
