Analysis of Changes in Broiler Microbiome Biodiversity Parameters Due to Intake of Glyphosate and Probiotic Bacillus Sp. Gl-8 Using Next-Generation Sequencing
Abstract. In recent years, there have been more data that the nonselective herbicide glyphosate (GLY) can negatively impact gut bacterial communities. The aim of our study was to investigate the composition of broiler caecal microbiome under chronic exposure to GLY and the introduction of a probiotic microorganism strain into the diet. 120 broilers were divided into three groups: Group 1 of control birds fed the basic diet (BD); Group 2 of experimental birds fed BD supplemented with GLY; and Group 3 of experimental birds fed BD supplemented with GLY and a probiotic strain of the microorganism Bacillus sp. GL-8. For analysis, we used the next-generation sequencing (NGS) technique. Due to the GLY administration, there was a trend of lowering the biodiversity of normal microflora representatives, along with intestinal colonization by undesirable forms of microorganisms. In particular, when adding GLY (Group 2), we observed a decreased number of Tepidimicrobium representatives (0.001 ±0.00006%) that ferment indigestible polysaccharides, while in Group 1 their
